4HVW
Crystal structure of the T98E c-Src-SH3 domain mutant in complex with the high affinity peptide VSL12
Summary for 4HVW
| Entry DOI | 10.2210/pdb4hvw/pdb |
| Related | 1QWE 1QWF 1SRL 3FJ5 4HVU 4HVV |
| Descriptor | Proto-oncogene tyrosine-protein kinase Src, SYNTHETIC PEPTIDE Acetyl-VSLARRPLPPLP, ACETYL GROUP, ... (5 entities in total) |
| Functional Keywords | beta shandwich, sh3 like barrel, kinase, proline rich motifs, signaling protein-peptide complex, signaling protein/peptide |
| Biological source | Gallus gallus (bantam,chickens) More |
| Cellular location | Cell membrane (By similarity): P00523 |
| Total number of polymer chains | 2 |
| Total formula weight | 8446.35 |
| Authors | Camara-Artigas, A. (deposition date: 2012-11-07, release date: 2013-05-01, Last modification date: 2023-09-20) |
| Primary citation | Bacarizo, J.,Camara-Artigas, A. Atomic resolution structures of the c-Src SH3 domain in complex with two high-affinity peptides from classes I and II. Acta Crystallogr.,Sect.D, 69:756-766, 2013 Cited by PubMed Abstract: The atomic resolution crystal structures of complexes between the SH3 domain of the c-Src tyrosine kinase and two high-affinity peptides belonging to class I and class II have been solved. The crystals of the Thr98Asp and Thr98Glu mutants in complex with the APP12 peptide (APPLPPRNRPRL) belonged to the trigonal space group P3121 and in both cases the asymmetric unit was composed of one molecule of the SH3-APP12 complex. The crystals of the Thr98Glu mutant in complex with the VSL12 peptide (VSLARRPLPLP) belonged to the trigonal space group P3221 and the asymmetric unit was also composed of a single molecule of the SH3-VSL12 complex. All crystals were obtained in the presence of PEG 300 under the same conditions as reported for the intertwined dimeric structure of the c-Src SH3 domain, but the presence of the peptide stabilizes the monomeric form of the domain. These structures allow a detailed analysis of the role of salt bridges, cation-π interactions and hydrogen bonds in the binding of proline-rich motifs to the c-Src SH3 domain. Moreover, these crystallographic structures allow the role of water molecules in the binding of these motifs to the c-Src SH3 domain to be studied for the first time. PubMed: 23633584DOI: 10.1107/S0907444913001522 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (0.98 Å) |
Structure validation
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