4HVN
Crystal structure of hypothetical protein with ketosteroid isomerase-like protein fold from Catenulispora acidiphila DSM 44928 in complex with Trimethylamine.
Summary for 4HVN
| Entry DOI | 10.2210/pdb4hvn/pdb |
| Related | 4H3U |
| Descriptor | hypothetical protein, N,N-dimethylmethanamine (3 entities in total) |
| Functional Keywords | structural genomics, psi-biology, midwest center for structural genomics, mcsg, unknown function |
| Biological source | Catenulispora acidiphila |
| Total number of polymer chains | 2 |
| Total formula weight | 34728.95 |
| Authors | Filippova, E.V.,Minasov, G.,Shuvalova, L.,Kiryukhina, O.,Jedrzejczak, R.,Joachimiak, A.,Anderson, W.F.,Midwest Center for Structural Genomics (MCSG) (deposition date: 2012-11-06, release date: 2012-11-21, Last modification date: 2024-11-06) |
| Primary citation | Filippova, E.V.,Luan, C.H.,Dunne, S.F.,Kiryukhina, O.,Minasov, G.,Shuvalova, L.,Anderson, W.F. Structural characterization of a hypothetical protein: a potential agent involved in trimethylamine metabolism in Catenulispora acidiphila. J.Struct.Funct.Genom., 15:33-40, 2014 Cited by PubMed Abstract: Catenulispora acidiphila is a newly identified lineage of actinomycetes that produces antimicrobial activities and represents a promising source of novel antibiotics and secondary metabolites. Among the discovered protein coding genes, 68 % were assigned a putative function, while the remaining 32 % are genes encoding "hypothetical" proteins. Caci_0382 is one of the "hypothetical" proteins that has very few homologs. Sequence analysis shows that the protein belongs to the NTF2-like protein family. The structure of Caci_0382 demonstrates that it shares the same fold and has a similar active site as limonene-1,2-epoxide hydrolase, which suggests that it may have a related function. Using a fluorescence thermal shift assay, we identified stabilizing compounds that suggest potential natural ligands of Caci_0382. Using this information, we determined the crystal structure in complex with trimethylamine to provide a better understanding of the function of this uncharacterized protein. PubMed: 24562475DOI: 10.1007/s10969-014-9176-z PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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