4HPG

Crystal structure of a glycosylated beta-1,3-glucanase (HEV B 2), an allergen from Hevea brasiliensis

「3EM5」から置き換えられました

4HPG の概要

• エントリーDOI: 10.2210/pdb4hpg/pdb
• 分子名称: Beta-1,3-glucanase, alpha-L-fucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, DI(HYDROXYETHYL)ETHER, ... (5 entities in total)
• 機能のキーワード: allergen, glycoprotein, glycoside hydrolase, gh17 family, pathogenesis-related class-2 protein, tim barrel, glycosidase hydrolase, carbohydrate/sugar binding, glycosylation, pyroglutamic acid (n-terminal residue), latex, hydrolase
• 由来する生物種: Hevea brasiliensis (Para rubber tree,jebe,rubbertree,siringa)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 142444.33

構造登録者

Rodriguez-Romero, A.,Hernandez-Santoyo, A. (登録日: 2012-10-23, 公開日: 2013-11-27, 最終更新日: 2024-11-06)

主引用文献

Rodriguez-Romero, A.,Hernandez-Santoyo, A.,Fuentes-Silva, D.,Palomares, L.A.,Munoz-Cruz, S.,Yepez-Mulia, L.,Orozco-Martinez, S.
Structural analysis of the endogenous glycoallergen Hev b 2 (endo-beta-1,3-glucanase) from Hevea brasiliensis and its recognition by human basophils.
Acta Crystallogr.,Sect.D, 70:329-341, 2014

PubMed Abstract: Endogenous glycosylated Hev b 2 (endo-β-1,3-glucanase) from Hevea brasiliensis is an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. The carbohydrate moieties of Hev b 2 constitute a potentially important IgE-binding epitope that could be responsible for its cross-reactivity. Here, the structure of the endogenous isoform II of Hev b 2 that exhibits three post-translational modifications, including an N-terminal pyroglutamate and two glycosylation sites at Asn27 and at Asn314, is reported from two crystal polymorphs. These modifications form a patch on the surface of the molecule that is proposed to be one of the binding sites for IgE. A structure is also proposed for the most important N-glycan present in this protein as determined by digestion with specific enzymes. To analyze the role of the carbohydrate moieties in IgE antibody binding and in human basophil activation, the glycoallergen was enzymatically deglycosylated and evaluated. Time-lapse automated video microscopy of basophils stimulated with glycosylated Hev b 2 revealed basophil activation and degranulation. Immunological studies suggested that carbohydrates on Hev b 2 represent an allergenic IgE epitope. In addition, a dimer was found in each asymmetric unit that may reflect a regulatory mechanism of this plant defence protein.

PubMed: 24531467
DOI: 10.1107/S1399004713027673

実験手法

X-RAY DIFFRACTION (2.5364 Å)