4H8I

Structure of GluK2-LBD in complex with GluAzo

4H8I の概要

• エントリーDOI: 10.2210/pdb4h8i/pdb
• 関連するPDBエントリー: 4H8J
• 分子名称: Glutamate receptor, ionotropic kainate 2, (4R)-4-[(2E)-3-{4-[(E)-phenyldiazenyl]phenyl}prop-2-en-1-yl]-L-glutamic acid, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: membrane protein, glycoprotein, transmembrane protein, ligand-binding domain, ion transport, ion channel, ionotropic glutamate receptor, kainate receptor, gluazo, pcl, photochromic ligand, mes, synapse, presynaptic cell membrane, postsynaptic cell membrane, signaling protein
• 由来する生物種: Rattus norvegicus (rat); 詳細
• 細胞内の位置: Cell membrane ; Multi-pass membrane protein : P42260
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59437.42

構造登録者

Reiter, A.,Skerra, A.,Trauner, D.,Schiefner, A. (登録日: 2012-09-22, 公開日: 2013-09-25, 最終更新日: 2024-11-27)

主引用文献

Reiter, A.,Skerra, A.,Trauner, D.,Schiefner, A.
A photoswitchable neurotransmitter analogue bound to its receptor.
Biochemistry, 52:8972-8974, 2013

PubMed Abstract: Incorporation of the azobenzene derivative gluazo, a synthetic photochromic ligand, into a kainate receptor allows for the optical control of neuronal activity. The crystal structure of gluazo bound to a dimeric GluK2 ligand-binding domain reveals one monomer in a closed conformation, occupied by gluazo, and the other in an open conformation, with a bound buffer molecule. The glutamate group of gluazo interacts like the natural glutamate ligand, while its trans-azobenzene moiety protrudes into a tunnel. This elongated cavity presumably cannot accommodate a cis-azobenzene, which explains the reversible activation of the receptor upon photoisomerization.

PubMed: 24295282
DOI: 10.1021/bi4014402

実験手法

X-RAY DIFFRACTION (2 Å)