4H24

Cytochrome P450BM3-CIS cyclopropanation catalyst

4H24 の概要

• エントリーDOI: 10.2210/pdb4h24/pdb
• 関連するPDBエントリー: 4H23
• 分子名称: Cytochrome P450-BM3 variant P450BM3-Cis, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: cytochrome p450, enzymatic cyclopropanation, directed evolution, non-natural function, oxidoreductase
• 由来する生物種: Bacillus megaterium
• 細胞内の位置: Cytoplasm (By similarity): P14779
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 217419.23

構造登録者

Coelho, P.S.,Wang, Z.J.,Ener, M.E.,Baril, S.A.,Kannan, A.,Arnold, F.H.,Brustad, E.M. (登録日: 2012-09-11, 公開日: 2013-06-26, 最終更新日: 2023-09-20)

主引用文献

Coelho, P.S.,Wang, Z.J.,Ener, M.E.,Baril, S.A.,Kannan, A.,Arnold, F.H.,Brustad, E.M.
A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo.
Nat.Chem.Biol., 9:485-487, 2013

PubMed Abstract: Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting-state Fe(III)-to-Fe(II) reduction potential and substantially improves NAD(P)H-driven activity.

PubMed: 23792734
DOI: 10.1038/nchembio.1278

実験手法

X-RAY DIFFRACTION (2.5 Å)