4H24
Cytochrome P450BM3-CIS cyclopropanation catalyst
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Cytochrome P450-BM3 variant P450BM3-Cis | polymer | 470 | 53738.3 | 4 | UniProt (P14779) Pfam (PF00067) In PDB | Bacillus megaterium | Bifunctional P-450/NADPH-P450 reductase, Cytochrome P450(BM-3), Cytochrome P450BM-3, Cytochrome P450 102 |
2 | A, B, C, D | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 4 | Chemie (HEM) | |||
3 | water | water | 18.0 | 197 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 0 - 463 (UniProt: P14779)
PDB | External Database | Details |
---|---|---|
Ala 78 | Val 79 | engineered mutation |
Val 87 | Phe 88 | engineered mutation |
Ser 142 | Pro 143 | engineered mutation |
Ile 175 | Thr 176 | engineered mutation |
Val 184 | Ala 185 | engineered mutation |
Arg 226 | Ser 227 | engineered mutation |
Gln 236 | His 237 | engineered mutation |
Gly 252 | Glu 253 | engineered mutation |
Ala 268 | Thr 269 | engineered mutation |
Val 290 | Ala 291 | engineered mutation |
Val 353 | Leu 354 | engineered mutation |
Val 366 | Ile 367 | engineered mutation |
Lys 442 | Glu 443 | engineered mutation |
His 464 | - | expression tag |
His 465 | - | expression tag |
His 466 | - | expression tag |
His 467 | - | expression tag |
His 468 | - | expression tag |
His 469 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 214953.3 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 2465.9 | |
All* | Total formula weight | 217419.2 |