4H1L

TCR interaction with peptide mimics of nickel offers structural insights in nickel contact allergy

4H1L の概要

• エントリーDOI: 10.2210/pdb4h1l/pdb
• 関連するPDBエントリー: 4H25 4H26
• 分子名称: HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen, mimotope peptide, ... (5 entities in total)
• 機能のキーワード: protein-protein complex, immunoglobin fold, tcr recogniton of mhc, mhc ii, glycosidation, membrane, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein: P01903
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 138353.32

構造登録者

Kappler, J.W.,Yin, L.,Dai, S.,Marrack, P. (登録日: 2012-09-10, 公開日: 2012-11-14, 最終更新日: 2024-11-27)

主引用文献

Yin, L.,Crawford, F.,Marrack, P.,Kappler, J.W.,Dai, S.
T-cell receptor (TCR) interaction with peptides that mimic nickel offers insight into nickel contact allergy.
Proc.Natl.Acad.Sci.USA, 109:18517-18522, 2012

PubMed Abstract: T cell-mediated allergy to Ni(++) is one of the most common forms of allergic contact dermatitis, but how the T-cell receptor (TCR) recognizes Ni(++) is unknown. We studied a TCR from an allergic patient that recognizes Ni(++) bound to the MHCII molecule DR52c containing an unknown self-peptide. We identified mimotope peptides that can replace both the self-peptide and Ni(++) in this ligand. They share a p7 lysine whose εNH(2) group is surface-exposed when bound to DR52c. Whereas the TCR uses germ-line complementary-determining region (CDR)1/2 amino acids to dock in the conventional diagonal mode on the mimotope-DR52c complex, the interface is dominated by the TCR Vβ CDR3 interaction with the p7 lysine. Mutations in the TCR CDR loops have similar effects on the T-cell response to either the mimotope or Ni(++) ligand. We suggest that the mimotope p7 lysine mimics Ni(++) in the natural TCR ligand and that MHCII β-chain flexibility in the area around the peptide p7 position forms a common site for cation binding in metal allergies.

PubMed: 23091041
DOI: 10.1073/pnas.1215928109

実験手法

X-RAY DIFFRACTION (3.3 Å)