4GUR

Crystal structure of LSD2-NPAC with H3 in space group P21

4GUR の概要

• エントリーDOI: 10.2210/pdb4gur/pdb
• 関連するPDBエントリー: 4GU1 4GUS 4GUT
• 分子名称: Lysine-specific histone demethylase 1B, Putative oxidoreductase GLYR1, Histone H3.3, ... (7 entities in total)
• 機能のキーワード: histone demethylase, oxidoreductase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus (By similarity): Q8NB78; Nucleus: Q49A26 P84243
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 103917.35

構造登録者

Chen, F.,Dong, Z.,Fang, J.,Yang, Y.,Li, Z.,Xu, Y.,Yang, H.,Wang, P.,Fang, R.,Shi, Y.,Xu, Y. (登録日: 2012-08-29, 公開日: 2013-01-16, 最終更新日: 2023-11-08)

主引用文献

Fang, R.,Chen, F.,Dong, Z.,Hu, D.,Barbera, A.J.,Clark, E.A.,Fang, J.,Yang, Y.,Mei, P.,Rutenberg, M.,Li, Z.,Zhang, Y.,Xu, Y.,Yang, H.,Wang, P.,Simon, M.D.,Zhou, Q.,Li, J.,Marynick, M.P.,Li, X.,Lu, H.,Kaiser, U.B.,Kingston, R.E.,Xu, Y.,Shi, Y.G.
LSD2/KDM1B and its cofactor NPAC/GLYR1 endow a structural and molecular model for regulation of H3K4 demethylation
Mol.Cell, 49:558-570, 2013

PubMed Abstract: Dynamic regulation of histone methylation represents a fundamental epigenetic mechanism underlying eukaryotic gene regulation, yet little is known about how the catalytic activities of histone demethylases are regulated. Here, we identify and characterize NPAC/GLYR1 as an LSD2/KDM1b-specific cofactor that stimulates H3K4me1 and H3K4me2 demethylation. We determine the crystal structures of LSD2 alone and LSD2 in complex with the NPAC linker region in the absence or presence of histone H3 peptide, at resolutions of 2.9, 2.0, and 2.25 Å, respectively. These crystal structures and further biochemical characterization define a dodecapeptide of NPAC (residues 214-225) as the minimal functional unit for its cofactor activity and provide structural determinants and a molecular mechanism underlying the intrinsic cofactor activity of NPAC in stimulating LSD2-catalyzed H3K4 demethylation. Thus, these findings establish a model for how a cofactor directly regulates histone demethylation and will have a significant impact on our understanding of catalytic-activity-based epigenetic regulation.

PubMed: 23260659
DOI: 10.1016/j.molcel.2012.11.019

実験手法

X-RAY DIFFRACTION (2.506 Å)