4GSK

Crystal structure of an Atg7-Atg10 crosslinked complex

4GSK の概要

• エントリーDOI: 10.2210/pdb4gsk/pdb
• 関連するPDBエントリー: 4GSJ 4GSL
• 分子名称: Ubiquitin-like modifier-activating enzyme ATG7, Ubiquitin-like-conjugating enzyme ATG10, ZINC ION (3 entities in total)
• 機能のキーワード: ubiquitin-like protein activation enzyme, ubiquitin-like protein transfer enzyme, protein transport-ligase complex, protein transport/ligase
• 由来する生物種: Saccharomyces cerevisiae (yeast); 詳細
• 細胞内の位置: Cytoplasm: P38862; Preautophagosomal structure membrane ; Peripheral membrane protein : Q07879
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 179682.46

構造登録者

Kaiser, S.E.,Mao, K.,Taherbhoy, A.M.,Yu, S.,Olszewski, J.L.,Duda, D.M.,Kurinov, I.,Deng, A.,Fenn, T.D.,Klionsky, D.J.,Schulman, B.A. (登録日: 2012-08-27, 公開日: 2012-11-14, 最終更新日: 2023-09-13)

主引用文献

Kaiser, S.E.,Mao, K.,Taherbhoy, A.M.,Yu, S.,Olszewski, J.L.,Duda, D.M.,Kurinov, I.,Deng, A.,Fenn, T.D.,Klionsky, D.J.,Schulman, B.A.
Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures.
Nat.Struct.Mol.Biol., 19:1242-1249, 2012

PubMed Abstract: Core functions of autophagy are mediated by ubiquitin-like protein (UBL) cascades, in which a homodimeric E1 enzyme, Atg7, directs the UBLs Atg8 and Atg12 to their respective E2 enzymes, Atg3 and Atg10. Crystallographic and mutational analyses of yeast (Atg7-Atg3)(2) and (Atg7-Atg10)(2) complexes reveal noncanonical, multisite E1-E2 recognition in autophagy. Atg7's unique N-terminal domain recruits distinctive elements from the Atg3 and Atg10 'backsides'. This, along with E1 and E2 conformational variability, allows presentation of 'frontside' Atg3 and Atg10 active sites to the catalytic cysteine in the C-terminal domain from the opposite Atg7 protomer in the homodimer. Despite different modes of binding, the data suggest that common principles underlie conjugation in both noncanonical and canonical UBL cascades, whereby flexibly tethered E1 domains recruit E2s through surfaces remote from their active sites to juxtapose the E1 and E2 catalytic cysteines.

PubMed: 23142976
DOI: 10.1038/nsmb.2415

実験手法

X-RAY DIFFRACTION (2.9 Å)