4GPU

Crystal structure of K. lactis Dxo1 (YDR370C) in complex with manganese

4GPU の概要

• エントリーDOI: 10.2210/pdb4gpu/pdb
• 関連するPDBエントリー: 4GPS
• 分子名称: KLLA0E02245p, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: decapping, 5'-3' exoribonuclease, hydrolase
• 由来する生物種: Kluyveromyces lactis (Yeast)
• 細胞内の位置: Cytoplasm : Q6CPU0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48601.29

構造登録者

Chang, J.H.,Chiba, K.,Tong, L. (登録日: 2012-08-21, 公開日: 2012-09-12, 最終更新日: 2023-09-13)

主引用文献

Chang, J.H.,Jiao, X.,Chiba, K.,Oh, C.,Martin, C.E.,Kiledjian, M.,Tong, L.
Dxo1 is a new type of eukaryotic enzyme with both decapping and 5'-3' exoribonuclease activity.
Nat.Struct.Mol.Biol., 19:1011-1017, 2012

PubMed Abstract: Recent studies showed that Rai1 is a crucial component of the mRNA 5'-end-capping quality-control mechanism in yeast. The yeast genome encodes a weak homolog of Rai1, Ydr370C, but little is known about this protein. Here we report the crystal structures of Ydr370C from Kluyveromyces lactis and the first biochemical and functional studies on this protein. The overall structure of Ydr370C is similar to Rai1. Ydr370C has robust decapping activity on RNAs with unmethylated caps, but it has no detectable pyrophosphohydrolase activity. Unexpectedly, Ydr370C also possesses distributive, 5'-3' exoRNase activity, and we propose the name Dxo1 for this new eukaryotic enzyme with both decapping and exonuclease activities. Studies of yeast in which both Dxo1 and Rai1 are disrupted reveal that mRNAs with incomplete caps are produced even under normal growth conditions, in sharp contrast to current understanding of the capping process.

PubMed: 22961381
DOI: 10.1038/nsmb.2381

実験手法

X-RAY DIFFRACTION (2.8 Å)