4GN1

Crystal Structure of the RA and PH domains of Lamellipodin

4GN1 の概要

• エントリーDOI: 10.2210/pdb4gn1/pdb
• 関連するPDBエントリー: 4GMV
• 分子名称: Ras-associated and pleckstrin homology domains-containing protein 1, MALONATE ION (3 entities in total)
• 機能のキーワード: ra-ph, coiled-coil region, ras-association domain, pleckstrin homology domain, cytoskeletal protein, ena/vasp binding, cell migration, cell adhesion, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Cytoplasmic side: Q70E73
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 121420.04

構造登録者

Chang, Y.C.E.,Wu, J. (登録日: 2012-08-16, 公開日: 2012-11-28, 最終更新日: 2023-09-13)

主引用文献

Chang, Y.C.,Zhang, H.,Brennan, M.L.,Wu, J.
Crystal structure of Lamellipodin implicates diverse functions in actin polymerization and Ras signaling.
Protein Cell, 4:211-219, 2013

PubMed Abstract: The adapter protein Lamellipodin (Lpd) plays an important role in cell migration. In particular, Lpd mediates lamellipodia formation by regulating actin dynamics via interacting with Ena/VASP proteins. Its RA-PH tandem domain configuration suggests that like its paralog RIAM, Lpd may also mediate particular Ras GTPase signaling. We determined the crystal structures of the Lpd RA-PH domains alone and with an N-terminal coiled-coil region (cc-RA-PH). These structures reveal that apart from the anticipated coiled-coil interaction, Lpd may also oligomerize through a second intermolecular contact site. We then validated both oligomerization interfaces in solution by mutagenesis. A fluorescence-polarization study demonstrated that Lpd binds phosphoinositol with low affinity. Based on our crystallographic and biochemical data, we propose that Lpd and RIAM serve diverse functions: Lpd plays a predominant role in regulating actin polymerization, and its function in mediating Ras GTPase signaling is largely suppressed compared to RIAM.

PubMed: 23483482
DOI: 10.1007/s13238-013-2082-5

実験手法

X-RAY DIFFRACTION (2.4 Å)