4FZR
Crystal Structure of SsfS6, Streptomyces sp. SF2575 glycosyltransferase
Summary for 4FZR
| Entry DOI | 10.2210/pdb4fzr/pdb |
| Related | 4G2T |
| Descriptor | SsfS6 (2 entities in total) |
| Functional Keywords | structural genomics, psi-biology, protein structure initiative, enzyme discovery for natural product biosynthesis, natpro, glycosyltransferase, transferase |
| Biological source | Streptomyces sp. SF2575 |
| Total number of polymer chains | 1 |
| Total formula weight | 43225.00 |
| Authors | Wang, F.,Zhou, M.,Singh, S.,Bingman, C.A.,Thorson, J.S.,Phillips Jr., G.N.,Enzyme Discovery for Natural Product Biosynthesis (NatPro) (deposition date: 2012-07-07, release date: 2012-07-25, Last modification date: 2024-11-20) |
| Primary citation | Wang, F.,Zhou, M.,Singh, S.,Yennamalli, R.M.,Bingman, C.A.,Thorson, J.S.,Phillips, G.N. Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis. Proteins, 81:1277-1282, 2013 Cited by PubMed Abstract: The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 Å resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation. PubMed: 23526584DOI: 10.1002/prot.24289 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.397 Å) |
Structure validation
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