4FWY

F33Y CuB myoglobin (F33Y L29H F43H sperm whale myoglobin) with copper bound

Summary for 4FWY

• Entry DOI: 10.2210/pdb4fwy/pdb
• Related: 4FWX 4FWZ
• Descriptor: Myoglobin, PROTOPORPHYRIN IX CONTAINING FE, COPPER (II) ION, ... (4 entities in total)
• Functional Keywords: globin, oxidase, transport protein
• Biological source: Physeter catodon (Sperm whale)
• Total number of polymer chains: 1
• Total formula weight: 18201.13

Authors

Gao, Y.-G.,Robinson, H.,Petrik, I.D.,Miner, K.D.,Lu, Y. (deposition date: 2012-07-02, release date: 2012-07-18, Last modification date: 2023-09-13)

Primary citation

Miner, K.D.,Mukherjee, A.,Gao, Y.G.,Null, E.L.,Petrik, I.D.,Zhao, X.,Yeung, N.,Robinson, H.,Lu, Y.
A Designed Functional Metalloenzyme that Reduces O(2) to H(2) O with Over One Thousand Turnovers.
Angew.Chem.Int.Ed.Engl., 51:5589-5592, 2012

PubMed Abstract: [Image: see text] Rational design of functional enzymes with high turnovers is a significant challenge, especially those with complex active site and difficult reactions, such as in respiratory oxidases. Introducing 2 His and 1 Tyr into myoglobin resulted in designed enzymes that reduce O to HO with > 1000 turnovers and minimal release of reactive oxygen species. This also showed that presence and positioning of Tyr, not Cu, are critical for activity.

PubMed: 22539151
DOI: 10.1002/anie.201201981

Experimental method

X-RAY DIFFRACTION (1.8 Å)