4FL5

Crystal structure of human 14-3-3 sigma in complex with a Tau-protein peptide surrounding pS214

Summary for 4FL5

• Entry DOI: 10.2210/pdb4fl5/pdb
• Related: 3LW1 3MHR 3NKX 3P1N
• Descriptor: 14-3-3 protein sigma, Microtubule-associated protein tau, CHLORIDE ION, ... (7 entities in total)
• Functional Keywords: peptide binding protein, signaling protein
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: P31947; Cytoplasm, cytosol : P10636
• Total number of polymer chains: 4
• Total formula weight: 56069.04

Authors

Schumacher, B.,Ottmann, C. (deposition date: 2012-06-14, release date: 2013-12-11, Last modification date: 2017-06-07)

Primary citation

Joo, Y.,Schumacher, B.,Landrieu, I.,Bartel, M.,Smet-Nocca, C.,Jang, A.,Choi, H.S.,Jeon, N.L.,Chang, K.A.,Kim, H.S.,Ottmann, C.,Suh, Y.H.
Involvement of 14-3-3 in tubulin instability and impaired axon development is mediated by Tau.
FASEB J., 29:4133-4144, 2015

PubMed Abstract: 14-3-3 proteins act as adapters that exert their function by interacting with their various protein partners. 14-3-3 proteins have been implicated in a variety of human diseases including neurodegenerative diseases. 14-3-3 proteins have recently been reported to be abundant in the neurofibrillary tangles (NFTs) observed inside the neurons of brains affected by Alzheimer's disease (AD). These NFTs are mainly constituted of phosphorylated Tau protein, a microtubule-associated protein known to bind 14-3-3. Despite this indication of 14-3-3 protein involvement in the AD pathogenesis, the role of 14-3-3 in the Tauopathy remains to be clarified. In the present study, we shed light on the role of 14-3-3 proteins in the molecular pathways leading to Tauopathies. Overexpression of the 14-3-3σ isoform resulted in a disruption of the tubulin cytoskeleton and prevented neuritic outgrowth in neurons. NMR studies validated the phosphorylated residues pSer214 and pSer324 in Tau as the 2 primary sites for 14-3-3 binding, with the crystal structure of 14-3-3σ in complex with Tau-pSer214 and Tau-pSer324 revealing the molecular details of the interaction. These data suggest a rationale for a possible pharmacologic intervention of the Tau/14-3-3 interaction.

PubMed: 26103986
DOI: 10.1096/fj.14-265009

Experimental method

X-RAY DIFFRACTION (1.9 Å)