4FHB

Enhancing DHFR catalysis by binding of an allosteric regulator nanobody (Nb179)

Summary for 4FHB

Entry DOI	10.2210/pdb4fhb/pdb
Related	3K74
Descriptor	Dihydrofolate reductase, Nb179, FOLIC ACID, ... (4 entities in total)
Functional Keywords	nanobody, dihydrofolate reductase, allosteric activator, reductase, oxidoreductase-protein binding complex, oxidoreductase/protein binding
Biological source	Escherichia coli More
Total number of polymer chains	2
Total formula weight	34084.48
Authors	Oyen, D. (deposition date: 2012-06-06, release date: 2013-04-24, Last modification date: 2017-11-15)
Primary citation	Oyen, D.,Wechselberger, R.,Srinivasan, V.,Steyaert, J.,Barlow, J.N. Mechanistic analysis of allosteric and non-allosteric effects arising from nanobody binding to two epitopes of the dihyrofolate reductase of Escherichia coli. Biochim.Biophys.Acta, 1834:2147-2157, 2013 Cited by PubMed: 23911607 DOI: 10.1016/j.bbapap.2013.07.010 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.8 Å)

Structure validation