4FGH
S. aureus dihydrofolate reductase co-crystallized with ethyl-DAP isobutenyl-dihydrophthalazine inhibitor
Summary for 4FGH
| Entry DOI | 10.2210/pdb4fgh/pdb |
| Related | 3M08 4FGG |
| Descriptor | Dihydrofolate reductase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, (2E)-3-{5-[(2,4-diamino-6-ethylpyrimidin-5-yl)methyl]-2,3-dimethoxyphenyl}-1-[(1S)-1-(2-methylprop-1-en-1-yl)phthalazin-2(1H)-yl]prop-2-en-1-one, ... (5 entities in total) |
| Functional Keywords | oxidoreductase-inhibitor complex, oxidoreductase/inhibitor |
| Biological source | Staphylococcus aureus |
| Total number of polymer chains | 1 |
| Total formula weight | 20104.66 |
| Authors | Bourne, C.R.,Barrow, W.W. (deposition date: 2012-06-04, release date: 2013-01-09, Last modification date: 2024-02-28) |
| Primary citation | Nammalwar, B.,Bourne, C.R.,Bunce, R.A.,Wakeham, N.,Bourne, P.C.,Ramnarayan, K.,Mylvaganam, S.,Berlin, K.D.,Barrow, E.W.,Barrow, W.W. Inhibition of Bacterial Dihydrofolate Reductase by 6-Alkyl-2,4-diaminopyrimidines. Chemmedchem, 7:1974-1982, 2012 Cited by PubMed Abstract: (±)-6-Alkyl-2,4-diaminopyrimidine-based inhibitors of bacterial dihydrofolate reductase (DHFR) have been prepared and evaluated for biological potency against Bacillus anthracis and Staphylococcus aureus. Biological studies revealed attenuated activity relative to earlier structures lacking substitution at C6 of the diaminopyrimidine moiety, though minimum inhibitory concentration (MIC) values are in the 0.125-8 μg mL(-1) range for both organisms. This effect was rationalized from three- dimensional X-ray structure studies that indicate the presence of a side pocket containing two water molecules adjacent to the main binding pocket. Because of the hydrophobic nature of the substitutions at C6, the main interactions are with protein residues Leu 20 and Leu 28. These interactions lead to a minor conformational change in the protein, which opens the pocket containing these water molecules such that it becomes continuous with the main binding pocket. These water molecules are reported to play a critical role in the catalytic reaction, highlighting a new area for inhibitor expansion within the limited architectural variation at the catalytic site of bacterial DHFR. PubMed: 22930550DOI: 10.1002/cmdc.201200291 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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