4FEE
High-resolution structure of pyruvate oxidase in complex with reaction intermediate 2-hydroxyethyl-thiamin diphosphate carbanion-enamine, crystal B
Summary for 4FEE
Entry DOI | 10.2210/pdb4fee/pdb |
Related | 1POW 1POX 2EZ4 2EZ8 2EZ9 2EZT 2EZU 4FEG |
Descriptor | Pyruvate oxidase, FLAVIN-ADENINE DINUCLEOTIDE, GLYCEROL, ... (8 entities in total) |
Functional Keywords | carbanion, structure activity relationship, oxidation-reduction, umpolung, thiamine diphosphate, reaction intermediate, oxidoreductase |
Biological source | Lactobacillus plantarum |
Total number of polymer chains | 2 |
Total formula weight | 136064.78 |
Authors | Meyer, D.,Neumann, P.,Koers, E.,Sjuts, H.,Luedtke, S.,Sheldrick, G.M.,Ficner, R.,Tittmann, K. (deposition date: 2012-05-30, release date: 2012-06-20, Last modification date: 2023-11-15) |
Primary citation | Meyer, D.,Neumann, P.,Koers, E.,Sjuts, H.,Ludtke, S.,Sheldrick, G.M.,Ficner, R.,Tittmann, K. Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin. Proc.Natl.Acad.Sci.USA, 109:10867-10872, 2012 Cited by PubMed: 22730460DOI: 10.1073/pnas.1201280109 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.13 Å) |
Structure validation
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