4FEE

High-resolution structure of pyruvate oxidase in complex with reaction intermediate 2-hydroxyethyl-thiamin diphosphate carbanion-enamine, crystal B

4FEE の概要

• エントリーDOI: 10.2210/pdb4fee/pdb
• 関連するPDBエントリー: 1POW 1POX 2EZ4 2EZ8 2EZ9 2EZT 2EZU 4FEG
• 分子名称: Pyruvate oxidase, FLAVIN-ADENINE DINUCLEOTIDE, GLYCEROL, ... (8 entities in total)
• 機能のキーワード: carbanion, structure activity relationship, oxidation-reduction, umpolung, thiamine diphosphate, reaction intermediate, oxidoreductase
• 由来する生物種: Lactobacillus plantarum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 136064.78

構造登録者

Meyer, D.,Neumann, P.,Koers, E.,Sjuts, H.,Luedtke, S.,Sheldrick, G.M.,Ficner, R.,Tittmann, K. (登録日: 2012-05-30, 公開日: 2012-06-20, 最終更新日: 2023-11-15)

• 主引用文献: Meyer, D.,Neumann, P.,Koers, E.,Sjuts, H.,Ludtke, S.,Sheldrick, G.M.,Ficner, R.,Tittmann, K.; Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin.; Proc.Natl.Acad.Sci.USA, 109:10867-10872, 2012; PubMed: 22730460; DOI: 10.1073/pnas.1201280109

実験手法

X-RAY DIFFRACTION (1.13 Å)