4FE8
Crystal Structure of Htt36Q3H-EX1-X1-C1(Alpha)
Summary for 4FE8
| Entry DOI | 10.2210/pdb4fe8/pdb |
| Related | 3IO4 3IO6 3IOR 3IOT 3IOU 3IOV 3IOW 4FEB 4FEC 4FED |
| Descriptor | Maltose-binding periplasmic protein,Huntingtin, ZINC ION (3 entities in total) |
| Functional Keywords | alpha helix, loop, beta-strand hairpin, beta strand hairpin, disease protein, signaling protein |
| Biological source | Escherichia coli (strain K12) More |
| Cellular location | Cytoplasm: P42858 |
| Total number of polymer chains | 3 |
| Total formula weight | 152352.64 |
| Authors | |
| Primary citation | Kim, M. Beta conformation of polyglutamine track revealed by a crystal structure of Huntingtin N-terminal region with insertion of three histidine residues. Prion, 7:221-228, 2013 Cited by PubMed Abstract: Huntington disease is an autosomal-dominant neurodegenerative disorder caused by a polyglutamine (polyQ) expansion (> 35Q) in the first exon (EX1) of huntingtin protein (Htt). mHtt protein is thought to adopt one or more toxic conformation(s) that are involved in pathogenic interactions in cells . However, the structure of mHtt is not known. Here, we present a near atomic resolution structure of mHtt36Q-EX1. To facilitate crystallization, three histidine residues (3H) were introduced within the Htt36Q stretch resulting in the sequence of Q 7HQHQHQ 27. The Htt36Q3H region adopts α-helix, loop, β-hairpin conformations. Furthermore, we observed interactions between the backbone of the Htt36Q3H β-strand with the aromatic residues mimicking putative-toxic interactions with other proteins. Our findings support previous predictions that the expanded mHtt-polyQ region adopts a β-sheet structure. Detailed structural information about mHtt improves our understanding of the pathogenic mechanisms in HD and other polyQ expansion disorders and may form the basis for rational design of small molecules that target toxic conformations of disease-causing proteins. PubMed: 23370273DOI: 10.4161/pri.23807 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
Download full validation report
