4FD4

Crystal structure of mosquito arylalkylamine N-Acetyltransferase like 5b

Summary for 4FD4

• Entry DOI: 10.2210/pdb4fd4/pdb
• Related: 4FD5 4FD6 4FD7
• Descriptor: arylalkylamine N-Acetyltransferase like 5b, GLYCEROL (3 entities in total)
• Functional Keywords: gnat, transferase
• Biological source: Aedes aegypti (Yellowfever mosquito)
• Total number of polymer chains: 2
• Total formula weight: 48998.38

Authors

Han, Q.,Robinson, R.,Li, J. (deposition date: 2012-05-26, release date: 2012-06-27, Last modification date: 2024-04-03)

Primary citation

Han, Q.,Robinson, H.,Ding, H.,Christensen, B.M.,Li, J.
Evolution of insect arylalkylamine N-acetyltransferases: structural evidence from the yellow fever mosquito, Aedes aegypti.
Proc.Natl.Acad.Sci.USA, 109:11669-11674, 2012

PubMed Abstract: Arylalkylamine N-acetyltransferase (aaNAT) catalyzes the transacetylation from acetyl-CoA to arylalkylamines. aaNATs are involved in sclerotization and neurotransmitter inactivation in insects. Phyletic distribution analysis confirms three clusters of aaNAT-like sequences in insects: typical insect aaNAT, polyamine NAT-like aaNAT, and mosquito unique putative aaNAT (paaNAT). Here we studied three proteins: aaNAT2, aaNAT5b, and paaNAT7, each from a different cluster. aaNAT2, a protein from the typical insect aaNAT cluster, uses histamine as a substrate as well as the previously identified arylalkylamines. aaNAT5b, a protein from polyamine NAT -like aaNAT cluster, uses hydrazine and histamine as substrates. The crystal structure of aaNAT2 was determined using single-wavelength anomalous dispersion methods, and that of native aaNAT2, aaNAT5b and paaNAT7 was detected using molecular replacement techniques. All three aaNAT structures have a common fold core of GCN5-related N-acetyltransferase superfamily proteins, along with a unique structural feature: helix/helices between β3 and β4 strands. Our data provide a start toward a more comprehensive understanding of the structure-function relationship and physiology of aaNATs from the mosquito Aedes aegypti and serve as a reference for studying the aaNAT family of proteins from other insect species. The structures of three different types of aaNATs may provide targets for designing insecticides for use in mosquito control.

PubMed: 22753468
DOI: 10.1073/pnas.1206828109

Experimental method

X-RAY DIFFRACTION (1.95 Å)