4F8B

Crystal Structure of the Covalent Thioimide Intermediate of Unimodular Nitrile Reductase QueF

Summary for 4F8B

• Entry DOI: 10.2210/pdb4f8b/pdb
• Related: 4FGC
• Descriptor: NADPH-dependent 7-cyano-7-deazaguanine reductase, 2-amino-5-[(Z)-iminomethyl]-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one, MAGNESIUM ION, ... (6 entities in total)
• Functional Keywords: beta barrel, protein thioimide complex, pterin binding fold, tunnel fold, trna modification enzyme, 7-cyano-7-deazaguanine, nadph, oxidoreductase
• Biological source: Bacillus subtilis subsp. subtilis
• Cellular location: Cytoplasm : O31678
• Total number of polymer chains: 5
• Total formula weight: 98240.05

Authors

Stec, B.,Swairjo, M.A. (deposition date: 2012-05-17, release date: 2012-07-11, Last modification date: 2023-09-13)

• Primary citation: Chikwana, V.M.,Stec, B.,Lee, B.W.,de Crecy-Lagard, V.,Iwata-Reuyl, D.,Swairjo, M.A.; Structural basis of biological nitrile reduction.; J.Biol.Chem., 287:30560-30570, 2012; PubMed: 22787148; DOI: 10.1074/jbc.M112.388538

Experimental method

X-RAY DIFFRACTION (2.502 Å)