4F7U

The 6S snRNP assembly intermediate

4F7U の概要

• エントリーDOI: 10.2210/pdb4f7u/pdb
• 分子名称: Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein E, ... (8 entities in total)
• 機能のキーワード: assembly machine, splicing
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Cytoplasm, cytosol : P62315 P62317 P62305 P62321 P62309 A1ZAW5
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 141450.53

構造登録者

Grimm, C.,Pelz, J.P. (登録日: 2012-05-16, 公開日: 2013-01-30, 最終更新日: 2023-09-13)

主引用文献

Grimm, C.,Chari, A.,Pelz, J.P.,Kuper, J.,Kisker, C.,Diederichs, K.,Stark, H.,Schindelin, H.,Fischer, U.
Structural Basis of Assembly Chaperone- Mediated snRNP Formation.
Mol.Cell, 49:692-703, 2013

PubMed Abstract: Small nuclear ribonucleoproteins (snRNPs) represent key constituents of major and minor spliceosomes. snRNPs contain a common core, composed of seven Sm proteins bound to snRNA, which forms in a step-wise and factor-mediated reaction. The assembly chaperone pICln initially mediates the formation of an otherwise unstable pentameric Sm protein unit. This so-called 6S complex docks subsequently onto the SMN complex, which removes pICln and enables the transfer of pre-assembled Sm proteins onto snRNA. X-ray crystallography and electron microscopy was used to investigate the structural basis of snRNP assembly. The 6S complex structure identifies pICln as an Sm protein mimic, which enables the topological organization of the Sm pentamer in a closed ring. A second structure of 6S bound to the SMN complex components SMN and Gemin2 uncovers a plausible mechanism of pICln elimination and Sm protein activation for snRNA binding. Our studies reveal how assembly factors facilitate formation of RNA-protein complexes in vivo.

PubMed: 23333303
DOI: 10.1016/j.molcel.2012.12.009

実験手法

X-RAY DIFFRACTION (1.898 Å)