4EW0

mouse MBD4 glycosylase domain in complex with a G:5hmU (5-hydroxymethyluracil) mismatch

Summary for 4EW0

• Entry DOI: 10.2210/pdb4ew0/pdb
• Related: 1NGN 4EVV 4EW4
• Descriptor: Methyl-CpG-binding domain protein 4, DNA (5'-D(*TP*CP*AP*GP*CP*GP*CP*AP*TP*GP*G)-3'), DNA (5'-D(*CP*CP*AP*TP*GP*(5HU)P*GP*CP*TP*GP*A)-3'), ... (6 entities in total)
• Functional Keywords: 5-hydroxymethyluracil, deamination, 5-methylcytosine, active dna demethylation, helix-hairpin-helix, dna glycosylase, hydrolase-dna complex, hydrolase/dna
• Biological source: Mus musculus (mouse); More
• Cellular location: Nucleus: Q9Z2D7
• Total number of polymer chains: 3
• Total formula weight: 24547.51

Authors

Hashimoto, H.,Zhang, X.,Cheng, X. (deposition date: 2012-04-26, release date: 2012-07-11, Last modification date: 2023-09-13)

• Primary citation: Hashimoto, H.,Zhang, X.,Cheng, X.; Excision of thymine and 5-hydroxymethyluracil by the MBD4 DNA glycosylase domain: structural basis and implications for active DNA demethylation.; Nucleic Acids Res., 40:8276-8284, 2012; PubMed: 22740654; DOI: 10.1093/nar/gks628

Experimental method

X-RAY DIFFRACTION (2.39 Å)