4EKV

Streptavidin 8-aa-loop H127C mutein with reversible biotin binding

Summary for 4EKV

• Entry DOI: 10.2210/pdb4ekv/pdb
• Related: 1SWE
• Descriptor: Streptavidin, BIOTIN, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: beta-barrel, binding protein, biotin-binding, biotin-binding protein
• Biological source: Streptomyces avidinii
• Cellular location: Secreted: P22629
• Total number of polymer chains: 1
• Total formula weight: 16566.26

Authors

Barrette-Ng, I.H.,Honetschlaeger, C.,Wong, S.L.,Ng, K.K.S. (deposition date: 2012-04-09, release date: 2012-06-13, Last modification date: 2023-09-13)

• Primary citation: O'Sullivan, V.J.,Barrette-Ng, I.,Hommema, E.,Hermanson, G.T.,Schofield, M.,Wu, S.C.,Honetschlaeger, C.,Ng, K.K.,Wong, S.L.; Development of a tetrameric streptavidin mutein with reversible biotin binding capability: engineering a mobile loop as an exit door for biotin.; Plos One, 7:e35203-e35203, 2012; PubMed: 22536357; DOI: 10.1371/journal.pone.0035203

Experimental method

X-RAY DIFFRACTION (2 Å)