4EDL

Crystal structure of beta-parvin CH2 domain

4EDL の概要

• エントリーDOI: 10.2210/pdb4edl/pdb
• 関連するPDBエントリー: 4EDM 4EDN
• 分子名称: Beta-parvin, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: calponin homology domain, protein-protein interaction, ld motif, integrin signaling, focal adhesion, adaptor protein, paxillin and integrin-linked kinase, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell junction, focal adhesion: Q9HBI1
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 93284.98

構造登録者

Stiegler, A.L.,Draheim, K.M.,Li, X.,Chayen, N.E.,Calderwood, D.A.,Boggon, T.J. (登録日: 2012-03-27, 公開日: 2012-08-08, 最終更新日: 2024-02-28)

主引用文献

Stiegler, A.L.,Draheim, K.M.,Li, X.,Chayen, N.E.,Calderwood, D.A.,Boggon, T.J.
Structural basis for paxillin binding and focal adhesion targeting of beta-parvin.
J.Biol.Chem., 287:32566-32577, 2012

PubMed Abstract: β-Parvin is a cytoplasmic adaptor protein that localizes to focal adhesions where it interacts with integrin-linked kinase and is involved in linking integrin receptors to the cytoskeleton. It has been reported that despite high sequence similarity to α-parvin, β-parvin does not bind paxillin, suggesting distinct interactions and cellular functions for these two closely related parvins. Here, we reveal that β-parvin binds directly and specifically to leucine-aspartic acid repeat (LD) motifs in paxillin via its C-terminal calponin homology (CH2) domain. We present the co-crystal structure of β-parvin CH2 domain in complex with paxillin LD1 motif to 2.9 Å resolution and find that the interaction is similar to that previously observed between α-parvin and paxillin LD1. We also present crystal structures of unbound β-parvin CH2 domain at 2.1 Å and 2.0 Å resolution that show significant conformational flexibility in the N-terminal α-helix, suggesting an induced fit upon paxillin binding. We find that β-parvin has specificity for the LD1, LD2, and LD4 motifs of paxillin, with K(D) values determined to 27, 42, and 73 μM, respectively, by surface plasmon resonance. Furthermore, we show that proper localization of β-parvin to focal adhesions requires both the paxillin and integrin-linked kinase binding sites and that paxillin is important for early targeting of β-parvin. These studies provide the first molecular details of β-parvin binding to paxillin and help define the requirements for β-parvin localization to focal adhesions.

PubMed: 22869380
DOI: 10.1074/jbc.M112.367342

実験手法

X-RAY DIFFRACTION (2.1 Å)