4E9L

FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections

Replaces:  3TKV

Summary for 4E9L

• Entry DOI: 10.2210/pdb4e9l/pdb
• Descriptor: Attaching and effacing protein, pathogenesis factor (2 entities in total)
• Functional Keywords: invasin-like, bacterial immunoglobulin, pathogenic, vaccine, cell adhesion
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 42623.85

Authors

Spraggon, G.,Nesta, B.,Alteri, C.,Gomes Moriel, D.,Rosini, R.,Veggi, D.,Smith, S.,Bertoldi, I.,Pastorello, I.,Ferlenghi, I.,Fontana, M.R.,Frankel, G.,Mobley, H.L.T.,Rappuoli, R.,Pizza, M.,Serino, L.,Soriana, M. (deposition date: 2012-03-21, release date: 2012-04-25, Last modification date: 2023-09-13)

Primary citation

Nesta, B.,Spraggon, G.,Alteri, C.,Moriel, D.G.,Rosini, R.,Veggi, D.,Smith, S.,Bertoldi, I.,Pastorello, I.,Ferlenghi, I.,Fontana, M.R.,Frankel, G.,Mobley, H.L.,Rappuoli, R.,Pizza, M.,Serino, L.,Soriani, M.
FdeC, a novel broadly conserved Escherichia coli adhesin eliciting protection against urinary tract infections.
MBio, 3:-, 2012

PubMed Abstract: The increasing antibiotic resistance of pathogenic Escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. We recently identified, by subtractive reverse vaccinology, nine Escherichia coli antigens that protect mice from sepsis. In this study, we characterized one of them, ECOK1_0290, named FdeC (factor adherence E. coli) for its ability to mediate E. coli adhesion to mammalian cells and extracellular matrix. This adhesive propensity was consistent with the X-ray structure of one of the FdeC domains that shows a striking structural homology to Yersinia pseudotuberculosis invasin and enteropathogenic E. coli intimin. Confocal imaging analysis revealed that expression of FdeC on the bacterial surface is triggered by interaction of E. coli with host cells. This phenotype was also observed in bladder tissue sections derived from mice infected with an extraintestinal strain. Indeed, we observed that FdeC contributes to colonization of the bladder and kidney, with the wild-type strain outcompeting the fdeC mutant in cochallenge experiments. Finally, intranasal mucosal immunization with recombinant FdeC significantly reduced kidney colonization in mice challenged transurethrally with uropathogenic E. coli, supporting a role for FdeC in urinary tract infections.

PubMed: 22496310
DOI: 10.1128/mBio.00010-12

Experimental method

X-RAY DIFFRACTION (1.9 Å)