4E8R

Structure of Oceanobacillus iheyensis group II intron in a ligand-free state in the presence of Cs+ and Mg2+

4E8R の概要

• エントリーDOI: 10.2210/pdb4e8r/pdb
• 関連するPDBエントリー: 4E8K 4E8M 4E8N 4E8P 4E8Q 4E8T 4E8V
• 分子名称: Group IIC intron, MAGNESIUM ION, CESIUM ION, ... (5 entities in total)
• 機能のキーワード: ribozyme, metalloenzyme, self-splicing, retrotransposition, rna
• 由来する生物種: Oceanobacillus iheyensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 134048.80

構造登録者

Marcia, M.,Pyle, A.M. (登録日: 2012-03-20, 公開日: 2012-11-14, 最終更新日: 2023-09-13)

主引用文献

Marcia, M.,Pyle, A.M.
Visualizing Group II Intron Catalysis through the Stages of Splicing.
Cell(Cambridge,Mass.), 151:497-507, 2012

PubMed Abstract: Group II introns are self-splicing ribozymes that share a reaction mechanism and a common ancestor with the eukaryotic spliceosome, thereby providing a model system for understanding the chemistry of pre-mRNA splicing. Here we report 14 crystal structures of a group II intron at different stages of catalysis. We provide a detailed mechanism for the first step of splicing, we describe a reversible conformational change between the first and the second steps of splicing, and we present the ligand-free intron structure after splicing in an active state that corresponds to the retrotransposable form of the intron. During each reaction, the reactants are aligned and activated by a heteronuclear four-metal-ion center that contains a metal cluster and obligate monovalent cations, and they adopt a structural arrangement similar to that of protein endonucleases. Based on our data, we propose a model for the splicing cycle and show that it is applicable to the eukaryotic spliceosome.

PubMed: 23101623
DOI: 10.1016/j.cell.2012.09.033

実験手法

X-RAY DIFFRACTION (3.36 Å)