4DXT

Human SUN2 (AA 522-717)

4DXT の概要

• エントリーDOI: 10.2210/pdb4dxt/pdb
• 関連するPDBエントリー: 4DXR 4DXS
• 分子名称: SUN domain-containing protein 2, POTASSIUM ION (3 entities in total)
• 機能のキーワード: beta-sandwich, linc complex, structural protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus inner membrane; Single-pass type II membrane protein: Q9UH99
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22175.91

構造登録者

Sosa, B.,Schwartz, T.U. (登録日: 2012-02-28, 公開日: 2012-06-06, 最終更新日: 2024-11-06)

主引用文献

Sosa, B.A.,Rothballer, A.,Kutay, U.,Schwartz, T.U.
LINC Complexes Form by Binding of Three KASH Peptides to Domain Interfaces of Trimeric SUN Proteins.
Cell(Cambridge,Mass.), 149:1035-1047, 2012

PubMed Abstract: Linker of nucleoskeleton and cytoskeleton (LINC) complexes span the nuclear envelope and are composed of KASH and SUN proteins residing in the outer and inner nuclear membrane, respectively. LINC formation relies on direct binding of KASH and SUN in the perinuclear space. Thereby, molecular tethers are formed that can transmit forces for chromosome movements, nuclear migration, and anchorage. We present crystal structures of the human SUN2-KASH1/2 complex, the core of the LINC complex. The SUN2 domain is rigidly attached to a trimeric coiled coil that prepositions it to bind three KASH peptides. The peptides bind in three deep and expansive grooves formed between adjacent SUN domains, effectively acting as molecular glue. In addition, a disulfide between conserved cysteines on SUN and KASH covalently links both proteins. The structure provides the basis of LINC complex formation and suggests a model for how LINC complexes might arrange into higher-order clusters to enhance force-coupling.

PubMed: 22632968
DOI: 10.1016/j.cell.2012.03.046

実験手法

X-RAY DIFFRACTION (2.22 Å)