4DXT

Human SUN2 (AA 522-717)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-17
Detector	ADSC QUANTUM 315
Spacegroup name	H 3 2
Unit cell lengths	79.580, 79.580, 199.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.660 - 2.220
R-factor	0.175
Rwork	0.169
R-free	0.22600
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.914
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: dev_975))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.700	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.048	0.570
Number of reflections	12305
<I/σ(I)>	20	3.5
Completeness [%]	99.8	99.9
Redundancy	5.9	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	16% PEG3350, 0.2 M potassium thiocyanate, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.0