4DW1
Crystal structure of the ATP-gated P2X4 ion channel in the ATP-bound, open state at 2.8 Angstroms
Summary for 4DW1
| Entry DOI | 10.2210/pdb4dw1/pdb |
| Related | 4DW0 |
| Descriptor | P2X purinoceptor, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total) |
| Functional Keywords | ion channel, transport protein |
| Biological source | Danio rerio (leopard danio,zebra danio,zebra fish) |
| Cellular location | Membrane; Multi-pass membrane protein (By similarity): Q6NYR1 |
| Total number of polymer chains | 1 |
| Total formula weight | 40103.17 |
| Authors | Hattori, M.,Gouaux, E. (deposition date: 2012-02-24, release date: 2012-04-25, Last modification date: 2024-10-30) |
| Primary citation | Hattori, M.,Gouaux, E. Molecular mechanism of ATP binding and ion channel activation in P2X receptors. Nature, 485:207-212, 2012 Cited by PubMed Abstract: P2X receptors are trimeric ATP-activated ion channels permeable to Na+, K+ and Ca2+. The seven P2X receptor subtypes are implicated in physiological processes that include modulation of synaptic transmission, contraction of smooth muscle, secretion of chemical transmitters and regulation of immune responses. Despite the importance of P2X receptors in cellular physiology, the three-dimensional composition of the ATP-binding site, the structural mechanism of ATP-dependent ion channel gating and the architecture of the open ion channel pore are unknown. Here we report the crystal structure of the zebrafish P2X4 receptor in complex with ATP and a new structure of the apo receptor. The agonist-bound structure reveals a previously unseen ATP-binding motif and an open ion channel pore. ATP binding induces cleft closure of the nucleotide-binding pocket, flexing of the lower body β-sheet and a radial expansion of the extracellular vestibule. The structural widening of the extracellular vestibule is directly coupled to the opening of the ion channel pore by way of an iris-like expansion of the transmembrane helices. The structural delineation of the ATP-binding site and the ion channel pore, together with the conformational changes associated with ion channel gating, will stimulate development of new pharmacological agents. PubMed: 22535247DOI: 10.1038/nature11010 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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