4DVN

Crystal structure of the glycoprotein Erns from the pestivirus BVDV-1 in complex with 2'-UMP

4DVN の概要

• エントリーDOI: 10.2210/pdb4dvn/pdb
• 関連するPDBエントリー: 4DVK 4DVL 4DW3
• 分子名称: E(rns) glycoprotein, alpha-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: virus glycoprotein, t2 ribonuclease, viral protein
• 由来する生物種: Bovine viral diarrhea virus (BVDV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43918.88

構造登録者

Krey, T.,Bontems, F.,Vonrhein, C.,Vaney, M.-C.,Bricogne, G.,Ruemenapf, T.,Rey, F.A. (登録日: 2012-02-23, 公開日: 2012-05-23, 最終更新日: 2024-11-06)

主引用文献

Krey, T.,Bontems, F.,Vonrhein, C.,Vaney, M.C.,Bricogne, G.,Rumenapf, T.,Rey, F.A.
Crystal Structure of the Pestivirus Envelope Glycoprotein E(rns) and Mechanistic Analysis of Its Ribonuclease Activity.
Structure, 20:862-873, 2012

PubMed Abstract: Pestiviruses, which belong to the Flaviviridae family of RNA viruses, are important agents of veterinary diseases causing substantial economical losses in animal farming worldwide. Pestivirus particles display three envelope glycoproteins at their surface: E(rns), E1, and E2. We report here the crystal structure of the catalytic domain of E(rns), the ribonucleolytic activity of which is believed to counteract the innate immunity of the host. The structure reveals a three-dimensional fold corresponding to T2 ribonucleases from plants and fungi. Cocrystallization experiments with mono- and oligonucleotides revealed the structural basis for substrate recognition at two binding sites previously identified for T2 RNases. A detailed analysis of poly-U cleavage products using (31)P-NMR and size exclusion chromatography, together with molecular docking studies, provides a comprehensive mechanistic picture of E(rns) activity on its substrates and reveals the presence of at least one additional nucleotide binding site.

PubMed: 22579253
DOI: 10.1016/j.str.2012.03.018

実験手法

X-RAY DIFFRACTION (2.38 Å)