4DL9

Crystal structure of S-nitrosoglutathione reductase from tomato (Solanum lycopersicum) in complex with NAD+

4DL9 の概要

• エントリーDOI: 10.2210/pdb4dl9/pdb
• 関連するPDBエントリー: 4DLA 4DLB
• 分子名称: Alcohol dehydrogenase class III, ZINC ION, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (6 entities in total)
• 機能のキーワード: rossmann fold, oxidoreductase, alcohol dehydrogenase class-3, s-nitrosoglutathione, nad+
• 由来する生物種: Solanum lycopersicum (tomato)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 87330.34

構造登録者

Kopecny, D.,Tylichova, M.,Briozzo, P. (登録日: 2012-02-06, 公開日: 2012-12-26, 最終更新日: 2023-09-13)

主引用文献

Kubienova, L.,Kopecny, D.,Tylichova, M.,Briozzo, P.,Skopalova, J.,Sebela, M.,Navratil, M.,Tache, R.,Luhova, L.,Barroso, J.B.,Petrivalsky, M.
Structural and functional characterization of a plant S-nitrosoglutathione reductase from Solanum lycopersicum.
Biochimie, 95:889-902, 2013

PubMed Abstract: S-nitrosoglutathione reductase (GSNOR), also known as S-(hydroxymethyl)glutathione (HMGSH) dehydrogenase, belongs to the large alcohol dehydrogenase superfamily, namely to the class III ADHs. GSNOR catalyses the oxidation of HMGSH to S-formylglutathione using a catalytic zinc and NAD(+) as a coenzyme. The enzyme also catalyses the NADH-dependent reduction of S-nitrosoglutathione (GSNO). In plants, GSNO has been suggested to serve as a nitric oxide (NO) reservoir locally or possibly as NO donor in distant cells and tissues. NO and NO-related molecules such as S-nitrosothiols (S-NOs) play a central role in the regulation of normal plant physiological processes and host defence. The enzyme thus participates in the cellular homeostasis of S-NOs and in the metabolism of reactive nitrogen species. Although GSNOR has recently been characterized from several organisms, this study represents the first detailed biochemical and structural characterization of a plant GSNOR, that from tomato (Solanum lycopersicum). SlGSNOR gene expression is higher in roots and stems compared to leaves of young plants. It is highly expressed in the pistil and stamens and in fruits during ripening. The enzyme is a dimer and preferentially catalyses reduction of GSNO while glutathione and S-methylglutathione behave as non-competitive inhibitors. Using NAD(+), the enzyme oxidizes HMGSH and other alcohols such as cinnamylalcohol, geraniol and ω-hydroxyfatty acids. The crystal structures of the apoenzyme, of the enzyme in complex with NAD(+) and in complex with NADH, solved up to 1.9 Å resolution, represent the first structures of a plant GSNOR. They confirm that the binding of the coenzyme is associated with the active site zinc movement and changes in its coordination. In comparison to the well characterized human GSNOR, plant GSNORs exhibit a difference in the composition of the anion-binding pocket, which negatively influences the affinity for the carboxyl group of ω-hydroxyfatty acids.

PubMed: 23274177
DOI: 10.1016/j.biochi.2012.12.009

実験手法

X-RAY DIFFRACTION (1.85 Å)