4DKX

Crystal Structure of the Rab 6A'(Q72L)

4DKX の概要

• エントリーDOI: 10.2210/pdb4dkx/pdb
• 分子名称: Ras-related protein Rab-6A, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: gtp binding fold, membrane trafficking, gtp, cytosol, protein transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Golgi apparatus membrane; Lipid-anchor: P20340
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50200.80

構造登録者

Park, H.H.,Shin, Y.-C. (登録日: 2012-02-04, 公開日: 2012-10-17, 最終更新日: 2024-03-20)

主引用文献

Shin, Y.-C.,Yoon, J.H.,Jang, T.-H.,Kim, S.Y.,Heo, W.D.,So, I.,Jeon, J.-H.,Park, H.H.
Crystal structure of Rab6A'(Q72L) mutant reveals unexpected GDP/Mg2+ binding with opened GTP-binding domain
Biochem.Biophys.Res.Commun., 424:269-273, 2012

PubMed Abstract: The Ras small G protein-superfamily is a family of GTP hydrolases whose activity is regulated by GTP/GDP binding states. Rab6A, a member of the Ras superfamily, is involved in the regulation of vesicle trafficking, which is critical for endocytosis, biosynthesis, secretion, cell differentiation and cell growth. Rab6A exists in two isoforms, termed RabA and Rab6A'. Substitution of Gln72 to Leu72 (Q72L) at Rab6 family blocks GTP hydrolysis activity and this mutation usually causes the Rab6 protein to be constitutively in an active form. Here, we report the crystal structure of the human Rab6A'(Q72L) mutant form at 1.9Å resolution. Unexpectedly, we found that Rab6A'(Q72L) possesses GDP/Mg(2+) in the GTP binding pockets, which is formed by a flexible switch I and switch II. Large conformational changes were also detected in the switch I and switch II regions. Our structure revealed that the non-hydrolysable, constitutively active form of Rab6A' can accommodate GDP/Mg(2+) in the open conformation.

PubMed: 22750005
DOI: 10.1016/j.bbrc.2012.06.102

実験手法

X-RAY DIFFRACTION (1.9 Å)