4DJB

A Structural Basis for the Assembly and Functions of a Viral Polymer that Inactivates Multiple Tumor Suppressors

Summary for 4DJB

• Entry DOI: 10.2210/pdb4djb/pdb
• Descriptor: E4-ORF3 (2 entities in total)
• Functional Keywords: adenovirus protein, rrm-like fold, hpv e2 dbd-like protein, tumor suppressor inactivation, nucleus, viral protein
• Biological source: Human adenovirus 5 (HAdV-5)
• Total number of polymer chains: 2
• Total formula weight: 30301.06

Authors

Ou, H.D.,Kwiatkowski, W.,Deerinck, T.J.,Noske, A.,Blain, K.Y.,Land, H.S.,Soria, C.,Powers, C.J.,May, A.P.,Shu, X.,Tsien, R.Y.,Fitzpatrick, J.A.J.,Long, J.A.,Ellisman, M.H.,Choe, S.,O'Shea, C.C. (deposition date: 2012-02-01, release date: 2012-10-31, Last modification date: 2024-11-20)

Primary citation

Ou, H.D.,Kwiatkowski, W.,Deerinck, T.J.,Noske, A.,Blain, K.Y.,Land, H.S.,Soria, C.,Powers, C.J.,May, A.P.,Shu, X.,Tsien, R.Y.,Fitzpatrick, J.A.,Long, J.A.,Ellisman, M.H.,Choe, S.,O'Shea, C.C.
A Structural Basis for the Assembly and Functions of a Viral Polymer that Inactivates Multiple Tumor Suppressors.
Cell(Cambridge,Mass.), 151:304-319, 2012

PubMed Abstract: Evolution of minimal DNA tumor virus' genomes has selected for small viral oncoproteins that hijack critical cellular protein interaction networks. The structural basis for the multiple and dominant functions of adenovirus oncoproteins has remained elusive. E4-ORF3 forms a nuclear polymer and simultaneously inactivates p53, PML, TRIM24, and MRE11/RAD50/NBS1 (MRN) tumor suppressors. We identify oligomerization mutants and solve the crystal structure of E4-ORF3. E4-ORF3 forms a dimer with a central β core, and its structure is unrelated to known polymers or oncogenes. E4-ORF3 dimer units coassemble through reciprocal and nonreciprocal exchanges of their C-terminal tails. This results in linear and branched oligomer chains that further assemble in variable arrangements to form a polymer network that partitions the nuclear volume. E4-ORF3 assembly creates avidity-driven interactions with PML and an emergent MRN binding interface. This reveals an elegant structural solution whereby a small protein forms a multivalent matrix that traps disparate tumor suppressors.

PubMed: 23063122
DOI: 10.1016/j.cell.2012.08.035

Experimental method

X-RAY DIFFRACTION (2.053 Å)