4D5N

Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state

Summary for 4D5N

Related4D5L 4D5Y 4D61 4D66 4D67 4D68
EMDB information2810
DescriptorEUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1, CRICKET PARALYSIS VIRUS IRES RNA (2 entities in total)
Functional Keywordsribosome-rna complex, crpv ires, ribosome, termination, release factors, ribosome/rna
Biological sourceHOMO SAPIENS (HUMAN)
More
Cellular locationCytoplasm P62495
Total number of polymer chains2
Total molecular weight113404.62
Authors
Muhs, M.,Hilal, T.,Mielke, T.,Skabkin, M.A.,Sanbonmatsu, K.Y.,Pestova, T.V.,Spahn, C.M.T. (deposition date: 2014-11-06, release date: 2015-02-04, Last modification date: 2017-08-30)
Primary citation
Muhs, M.,Hilal, T.,Mielke, T.,Skabkin, M.A.,Sanbonmatsu, K.Y.,Pestova, T.V.,Spahn, C.M.T.
Cryo-Em of Ribosomal 80S Complexes with Termination Factors Reveals the Translocated Cricket Paralysis Virus Ires.
Mol.Cell, 57:422-, 2015
PubMed: 25601755 (PDB entries with the same primary citation)
DOI: 10.1016/J.MOLCEL.2014.12.016
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY (9 Å)
?

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone260.9%4.5%0.46MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures