4CYS

G6 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid

Summary for 4CYS

Entry DOI	10.2210/pdb4cys/pdb
Related	4CXK 4CXS 4CXU 4CYR
Descriptor	ARYLSULFATASE, CALCIUM ION, phenylphosphonic acid, ... (6 entities in total)
Functional Keywords	hydrolase, catalytic promiscuity, directed evolution, neutral drift, sulfatase, superfamily
Biological source	PSEUDOMONAS AERUGINOSA
Total number of polymer chains	2
Total formula weight	120785.51
Authors	Miton, C.M.,Jonas, S.,Mohammed, M.F.,Fischer, G.,Loo, B.v.,Kintses, B.,Hyvonen, M.,Tokuriki, N.,Hollfelder, F. (deposition date: 2014-04-14, release date: 2015-04-29, Last modification date: 2023-12-20)
Primary citation	Miton, C.M.,Jonas, S.,Fischer, G.,Duarte, F.,Mohamed, M.F.,van Loo, B.,Kintses, B.,Kamerlin, S.C.L.,Tokuriki, N.,Hyvonen, M.,Hollfelder, F. Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset. Proc. Natl. Acad. Sci. U.S.A., 115:E7293-E7302, 2018 Cited by PubMed: 30012610 DOI: 10.1073/pnas.1607817115 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.88 Å)

Structure validation