4CYK

Structural basis for binding of Pan3 to Pan2 and its function in mRNA recruitment and deadenylation

Summary for 4CYK

• Entry DOI: 10.2210/pdb4cyk/pdb
• NMR Information: BMRB: 19959
• Descriptor: PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUNIT PAN3, ZINC ION (2 entities in total)
• Functional Keywords: transcription, pan3p, pan2p, polya, rna, deadenylation
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• Cellular location: Cytoplasm : P36102
• Total number of polymer chains: 1
• Total formula weight: 4806.81

Authors

Wolf, J.,Valkov, E.,Allen, M.D.,Meineke, B.,Gordiyenko, Y.,McLaughlin, S.H.,Olsen, T.M.,Robinson, C.V.,Bycroft, M.,Stewart, M.,Passmore, L.A. (deposition date: 2014-04-11, release date: 2014-05-21, Last modification date: 2024-05-15)

Primary citation

Wolf, J.,Valkov, E.,Allen, M.D.,Meineke, B.,Gordiyenko, Y.,Mclaughlin, S.H.,Olsen, T.M.,Robinson, C.V.,Bycroft, M.,Stewart, M.,Passmore, L.A.
Structural Basis for Pan3 Binding to Pan2 and its Function in Mrna Recruitment and Deadenylation.
Embo J., 33:1514-, 2014

PubMed Abstract: The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability. Although the exonuclease activity resides in Pan2, efficient deadenylation requires Pan3. The mechanistic role of Pan3 is unclear. Here, we show that Pan3 binds RNA directly both through its pseudokinase/C-terminal domain and via an N-terminal zinc finger that binds polyA RNA specifically. In contrast, isolated Pan2 is unable to bind RNA. Pan3 binds to the region of Pan2 that links its N-terminal WD40 domain to the C-terminal part that contains the exonuclease, with a 2:1 stoichiometry. The crystal structure of the Pan2 linker region bound to a Pan3 homodimer shows how the unusual structural asymmetry of the Pan3 dimer is used to form an extensive high-affinity interaction. This binding allows Pan3 to supply Pan2 with substrate polyA RNA, facilitating efficient mRNA deadenylation by the intact Pan2-Pan3 complex.

PubMed: 24872509
DOI: 10.15252/EMBJ.201488373

Experimental method

SOLUTION NMR