4CXG

Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement

4CXG の概要

• エントリーDOI: 10.2210/pdb4cxg/pdb
• 関連するPDBエントリー: 4CXB 4CXC 4CXD 4CXE 4CXH
• EMDBエントリー: 2623
• 分子名称: 18S RRNA - H44, PHENYLALANINE, 28S RRNA - H89, ... (10 entities in total)
• 機能のキーワード: translation, mammalian 80s ribosome, elongation cycle, trna selection, eukaryotic ternary complex
• 由来する生物種: ORYCTOLAGUS CUNICULUS (RABBIT); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 184578.74

構造登録者

Budkevich, T.V.,Giesebrecht, J.,Behrmann, E.,Loerke, J.,Ramrath, D.J.F.,Mielke, T.,Ismer, J.,Hildebrand, P.,Tung, C.-S.,Nierhaus, K.H.,Sanbonmatsu, K.Y.,Spahn, C.M.T. (登録日: 2014-04-07, 公開日: 2014-07-16, 最終更新日: 2024-05-08)

主引用文献

Budkevich, T.V.,Giesebrecht, J.,Behrmann, E.,Loerke, J.,Ramrath, D.J.,Mielke, T.,Ismer, J.,Hildebrand, P.W.,Tung, C.S.,Nierhaus, K.H.,Sanbonmatsu, K.Y.,Spahn, C.M.
Regulation of the Mammalian Elongation Cycle by Subunit Rolling: A Eukaryotic-Specific Ribosome Rearrangement.
Cell(Cambridge,Mass.), 158:121-, 2014

PubMed Abstract: The extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution cryoelectron microscopy maps of the mammalian 80S ribosome in the posttranslocational state and in complex with the eukaryotic eEF1A⋅Val-tRNA⋅GMPPNP ternary complex, revealing significant differences in the elongation mechanism between bacteria and mammals. Surprisingly, and in contrast to bacterial ribosomes, a rotation of the small subunit around its long axis and orthogonal to the well-known intersubunit rotation distinguishes the posttranslocational state from the classical pretranslocational state ribosome. We term this motion "subunit rolling." Correspondingly, a mammalian decoding complex visualized in substates before and after codon recognition reveals structural distinctions from the bacterial system. These findings suggest how codon recognition leads to GTPase activation in the mammalian system and demonstrate that in mammalia subunit rolling occurs during tRNA selection.

PubMed: 24995983
DOI: 10.1016/J.CELL.2014.04.044

実験手法

ELECTRON MICROSCOPY (8.7 Å)