4CSG

Structural insights into Toscana virus RNA encapsidation

4CSG の概要

• エントリーDOI: 10.2210/pdb4csg/pdb
• 関連するPDBエントリー: 4CSF 4CSL
• 分子名称: NUCLEOPROTEIN (2 entities in total)
• 機能のキーワード: viral protein, infectious
• 由来する生物種: TOSCANA VIRUS; 詳細
• 細胞内の位置: Virion: P21701 P21701
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 332895.34

構造登録者

Olal, D.,Daumke, O. (登録日: 2014-03-07, 公開日: 2014-04-09, 最終更新日: 2023-12-20)

主引用文献

Olal, D.,Dick, A.,Woods, V.L.,Liu, T.,Li, S.,Devignot, S.,Weber, F.,Saphire, E.O.,Daumke, O.
Structural Insights Into RNA Encapsidation and Helical Assembly of the Toscana Virus Nucleoprotein.
Nucleic Acids Res., 42:6025-, 2014

PubMed Abstract: Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses.

PubMed: 24688060
DOI: 10.1093/NAR/GKU229

実験手法

X-RAY DIFFRACTION (3.32 Å)