4CSF
Structural insights into Toscana virus RNA encapsidation
Summary for 4CSF
| Entry DOI | 10.2210/pdb4csf/pdb |
| Related | 4CSG |
| Descriptor | NUCLEOPROTEIN, RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3'), ... (5 entities in total) |
| Functional Keywords | viral protein-rna complex, viral protein, nucleocapsid, nucleoprotein, hexameric, infectious, viral protein/rna |
| Biological source | TOSCANA VIRUS More |
| Total number of polymer chains | 36 |
| Total formula weight | 699159.15 |
| Authors | Olal, D.,Daumke, O. (deposition date: 2014-03-07, release date: 2014-04-09, Last modification date: 2024-05-08) |
| Primary citation | Olal, D.,Dick, A.,Woods, V.L.,Liu, T.,Li, S.,Devignot, S.,Weber, F.,Saphire, E.O.,Daumke, O. Structural Insights Into RNA Encapsidation and Helical Assembly of the Toscana Virus Nucleoprotein. Nucleic Acids Res., 42:6025-, 2014 Cited by PubMed Abstract: Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses. PubMed: 24688060DOI: 10.1093/NAR/GKU229 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.598 Å) |
Structure validation
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