4CS0

Direct visualisation of strain-induced protein post-translational modification

4CS0 の概要

• エントリーDOI: 10.2210/pdb4cs0/pdb
• 関連するPDBエントリー: 4CRY 4CRZ
• 分子名称: ASPARTATE 1-DECARBOXYLASE, PANZ, THIOCYANATE ION, ... (6 entities in total)
• 機能のキーワード: lyase, coenzyme a, radiation damage, pantothenate
• 由来する生物種: ESCHERICHIA COLI K-12; 詳細
• 細胞内の位置: Cytoplasm : P0A790
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32410.66

構造登録者

Monteiro, D.C.F.,Patel, V.,Bartlett, C.P.,Grant, T.D.,Nozaki, S.,Gowdy, J.A.,Snell, E.H.,Niki, H.,Pearson, A.R.,Webb, M.E. (登録日: 2014-03-02, 公開日: 2015-03-25, 最終更新日: 2024-11-20)

主引用文献

Monteiro, D.C.,Patel, V.,Bartlett, C.P.,Nozaki, S.,Grant, T.D.,Gowdy, J.A.,Thompson, G.S.,Kalverda, A.P.,Snell, E.H.,Niki, H.,Pearson, A.R.,Webb, M.E.
The Structure of the Pand/Panz Protein Complex Reveals Negative Feedback Regulation of Pantothenate Biosynthesis by Coenzyme A.
Chem.Biol., 22:492-, 2015

PubMed Abstract: Coenzyme A (CoA) is an ubiquitous and essential cofactor, synthesized from the precursor pantothenate. Vitamin biosynthetic pathways are normally tightly regulated, including the pathway from pantothenate to CoA. However, no regulation of pantothenate biosynthesis has been identified. We have recently described an additional component in the pantothenate biosynthetic pathway, PanZ, which promotes the activation of the zymogen, PanD, to form aspartate α-decarboxylase (ADC) in a CoA-dependent manner. Here we report the structure of PanZ in complex with PanD, which reveals the structural basis for the CoA dependence of this interaction and activation. In addition, we show that PanZ acts as a CoA-dependent inhibitor of ADC catalysis. This inhibitory effect can effectively regulate the biosynthetic pathway to pantothenate, and thereby also regulate CoA biosynthesis. This represents a previously unobserved mode of metabolic regulation whereby a cofactor-utilizing protein negatively regulates the biosynthesis of the same cofactor.

PubMed: 25910242
DOI: 10.1016/J.CHEMBIOL.2015.03.017

実験手法

X-RAY DIFFRACTION (2.1 Å)