4CNO
Structure of the Salmonella typhi Type I dehydroquinase inhibited by a 3-dehydroquinic acid derivative
Summary for 4CNO
| Entry DOI | 10.2210/pdb4cno/pdb |
| Related | 4CNN 4CNP |
| Descriptor | 3-DEHYDROQUINATE DEHYDRATASE, (2R)-2-METHYL-3-DEHYDROQUINIC ACID (3 entities in total) |
| Functional Keywords | lyase, inhibitor, protein binding, shikimis acid pathway, substrate specificity |
| Biological source | SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHI |
| Total number of polymer chains | 4 |
| Total formula weight | 111540.68 |
| Authors | Otero, J.M.,Llamas-Saiz, A.L.,Maneiro, M.,Peon, A.,Lence, E.,Lamb, H.,Hawkins, A.R.,Gonzalez-Bello, C.,van Raaij, M.J. (deposition date: 2014-01-23, release date: 2014-10-08, Last modification date: 2023-12-20) |
| Primary citation | Maneiro, M.,Peon, A.,Lence, E.,Otero, J.M.,Van Raaij, M.J.,Thompson, P.,Hawkins, A.R.,Gonzalez-Bello, C. Insights into substrate binding and catalysis in bacterial type I dehydroquinase. Biochem. J., 462:415-424, 2014 Cited by PubMed Abstract: Structural, biochemical and computational studies to study substrate binding and the role of the conserved residues of the DHQ1 (type I dehydroquinase) enzyme active site are reported in the present paper. The crystal structure of DHQ1 from Salmonella typhi in complex with (2R)-2-methyl-3-dehydroquinic acid, a substrate analogue, was solved at 1.5 Å. The present study reveals a previously unknown key role for conserved Glu46, Phe145 and Met205 and Gln236, Pro234 and Ala233 residues, with the latter three being located in the flexible substrate-covering loop. Gln236 was shown to be responsible for the folding of this loop and for the dramatic reduction of its flexibility, which triggers active site closure. Glu46 was found to be key in bringing the substrate close to the lysine/histidine catalytic pocket to initiate catalysis. The present study could be useful in the rational design of inhibitors of this challenging and recognized target for the development of novel herbicides and antimicrobial agents. PubMed: 24957267DOI: 10.1042/BJ20140614 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.5 Å) |
Structure validation
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