4CL7
Crystal structure of VEGFR-1 domain 2 in presence of Cobalt
Summary for 4CL7
Entry DOI | 10.2210/pdb4cl7/pdb |
Related | 4CKV |
Descriptor | VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 1, COBALT (II) ION, 1,2-ETHANEDIOL, ... (4 entities in total) |
Functional Keywords | receptor, metal-binding |
Biological source | HOMO SAPIENS (HUMAN) |
Total number of polymer chains | 4 |
Total formula weight | 43827.24 |
Authors | Gaucher, J.-F.,Reille-Seroussi, M.,Gagey-Eilstein, N.,Broussy, S.,Coric, P.,Seijo, B.,Lascombe, M.-B.,Gautier, B.,Liu, W.-Q.,Huguenot, F.,Inguimbert, N.,Bouaziz, S.,Vidal, M.,Broutin, I. (deposition date: 2014-01-13, release date: 2015-01-28, Last modification date: 2023-12-20) |
Primary citation | Gaucher, J.-F.,Reille-Seroussi, M.,Gagey-Eilstein, N.,Broussy, S.,Coric, P.,Seijo, B.,Lascombe, M.-B.,Gautier, B.,Liu, W.-Q.,Huguenot, F.,Inguimbert, N.,Bouaziz, S.,Vidal, M.,Broutin, I. Biophysical Studies of the Induced Dimerization of Human Vegf R Receptor 1 Binding Domain by Divalent Metals Competing with Vegf-A Plos One, 11:67755-, 2016 Cited by PubMed Abstract: Angiogenesis is tightly regulated through the binding of vascular endothelial growth factors (VEGFs) to their receptors (VEGFRs). In this context, we showed that human VEGFR1 domain 2 crystallizes in the presence of Zn2+, Co2+ or Cu2+ as a dimer that forms via metal-ion interactions and interlocked hydrophobic surfaces. SAXS, NMR and size exclusion chromatography analyses confirm the formation of this dimer in solution in the presence of Co2+, Cd2+ or Cu2+. Since the metal-induced dimerization masks the VEGFs binding surface, we investigated the ability of metal ions to displace the VEGF-A binding to hVEGFR1: using a competition assay, we evidenced that the metals displaced the VEGF-A binding to hVEGFR1 extracellular domain binding at micromolar level. PubMed: 27942001DOI: 10.1371/JOURNAL.PONE.0167755 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
Download full validation report