4CG5

Cryo-EM of the Sec61-complex bound to the 80S ribosome translating a secretory substrate

Summary for 4CG5

Related4CG6 4CG7
EMDB information2511
DescriptorPROTEIN TRANSPORT PROTEIN SEC61 SUBUNIT ALPHA ISOFORM 1, PROTEIN TRANSPORT PROTEIN SEC61 SUBUNIT GAMMA, PROTEIN TRANSPORT PROTEIN SEC61 SUBUNIT BETA (3 entities in total)
Functional Keywordsprotein transport, ribosome, co-translational protein translocation
Biological sourceCANIS LUPUS FAMILIARIS (DOG)
Cellular locationEndoplasmic reticulum membrane ; Multi-pass membrane protein  P38377
Endoplasmic reticulum membrane; Single-pass membrane protein P60058 P60467
Total number of polymer chains3
Total molecular weight64024.5
Authors
Gogala, M.,Becker, T.,Beatrix, B.,Barrio-Garcia, C.,Berninghausen, O.,Beckmann, R. (deposition date: 2013-11-21, release date: 2014-02-05, Last modification date: 2017-08-30)
Primary citation
Gogala, M.,Becker, T.,Beatrix, B.,Armache, J.,Barrio-Garcia, C.,Berninghausen, O.,Beckmann, R.
Structures of the Sec61 Complex Engaged in Nascent Peptide Translocation or Membrane Insertion.
Nature, 506:107-, 2014
PubMed: 24499919 (PDB entries with the same primary citation)
DOI: 10.1038/NATURE12950
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY (7.4 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliers214.3%7.8%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 4cg5
no rotation
Molmil generated image of 4cg5
rotated about x axis by 90°
Molmil generated image of 4cg5
rotated about y axis by 90°