4CG5
Cryo-EM of the Sec61-complex bound to the 80S ribosome translating a secretory substrate
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | TVIPS TEMCAM-F416 (4k x 4k) |
| Number of Images | 9805 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.4 |
| Software | MAPPOS,Coot,MDFF,UCSF Chimera,Signature,SPIDER |
| Symmetry | C1 |
