4CFI

3D structure of FliC from Burkholderia pseudomallei

4CFI の概要

• エントリーDOI: 10.2210/pdb4cfi/pdb
• 分子名称: FLAGELLIN (2 entities in total)
• 機能のキーワード: structural protein, flagella, epitope, immune response
• 由来する生物種: BURKHOLDERIA PSEUDOMALLEI
• 細胞内の位置: Secreted : P70944
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25899.21

構造登録者

Lassaux, P.,Peri, C.,Ferrer-Navarro, M.,Gourlay, L.J.,Conchillo-Sole, O.,Daura, X.,Colombo, G.,Bolognesi, M. (登録日: 2013-11-18, 公開日: 2014-12-10, 最終更新日: 2023-12-20)

主引用文献

Nithichanon, A.,Rinchai, D.,Gori, A.,Lassaux, P.,Peri, C.,Conchillio-Sole, O.,Ferrer-Navarro, M.,Gourlay, L.J.,Nardini, M.,Vila, J.,Daura, X.,Colombo, G.,Bolognesi, M.,Lertmemonkolchai, G.
Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia Pseudomallei Flagellin.
Plos Negl Trop Dis, 9:03917-, 2015

PubMed Abstract: Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.

PubMed: 26222657
DOI: 10.1371/JOURNAL.PNTD.0003917

実験手法

X-RAY DIFFRACTION (1.3 Å)