4CC8

Pre-fusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy

Summary for 4CC8

• Entry DOI: 10.2210/pdb4cc8/pdb
• EMDB information: 2484
• Descriptor: GP41, GP120, MONOCLONAL ANTIBODY VRC03 FAB HEAVY CHAIN, ... (4 entities in total)
• Functional Keywords: viral protein-immune system complex, pre-fusion state, viral protein/immune system
• Biological source: HUMAN IMMUNODEFICIENCY VIRUS 1; More
• Total number of polymer chains: 12
• Total formula weight: 241174.68

Authors

Bartesaghi, A.,Merk, A.,Borgnia, M.J.,Milne, J.L.S.,Subramaniam, S. (deposition date: 2013-10-18, release date: 2013-10-30, Last modification date: 2024-11-20)

Primary citation

Bartesaghi, A.,Merk, A.,Borgnia, M.J.,Milne, J.L.S.,Subramaniam, S.
Prefusion Structure of Trimeric HIV-1 Envelope Glycoprotein Determined by Cryo-Electron Microscopy.
Nat.Struct.Mol.Biol., 20:1352-, 2013

PubMed Abstract: The activation of trimeric HIV-1 envelope glycoprotein (Env) by its binding to the cell-surface receptor CD4 and co-receptors (CCR5 or CXCR4) represents the first of a series of events that lead to fusion between viral and target-cell membranes. Here, we present the cryo-EM structure, at subnanometer resolution (~6 Å at 0.143 FSC), of the 'closed', prefusion state of trimeric HIV-1 Env complexed to the broadly neutralizing antibody VRC03. We show that three gp41 helices at the core of the trimer serve as an anchor around which the rest of Env is reorganized upon activation to the 'open' quaternary conformation. The architecture of trimeric HIV-1 Env in the prefusion state and in the activated intermediate state resembles the corresponding states of influenza hemagglutinin trimers, thus providing direct evidence for the similarity in entry mechanisms used by HIV-1, influenza and related enveloped viruses.

PubMed: 24154805
DOI: 10.1038/NSMB.2711

Experimental method

ELECTRON MICROSCOPY (6 Å)