4CC8
Pre-fusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 80 |
| Cs (mm) | 2.7 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 2000 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6 |
| Software | UCSF Chimera,EMAN,FREALIGN,IMAGIC |
| Symmetry | C3 |
