4CAS

Serial femtosecond crystallography structure of a photosynthetic reaction center

Summary for 4CAS

• Entry DOI: 10.2210/pdb4cas/pdb
• Descriptor: PHOTOSYNTHETIC REACTION CENTER CYTOCHROME C SUBUNIT, FE (II) ION, MENAQUINONE-7, ... (14 entities in total)
• Functional Keywords: photosynthesis, lipidic-sponge phase, reaction center, electron transport, cell membrane, metal- binding, transmembrane, chromophore
• Biological source: BLASTOCHLORIS VIRIDIS; More
• Total number of polymer chains: 4
• Total formula weight: 146387.98

Authors

Johansson, L.C.,Arnlund, D.,Katona, G.,White, T.A.,Barty, A.,DePonte, D.P.,Shoeman, R.L.,Wickstrand, C.,Sharma, A.,Williams, G.J.,Aquila, A.,Bogan, M.J.,Caleman, C.,Davidsson, J.,Doak, R.B.,Frank, M.,Fromme, R.,Galli, L.,Grotjohann, I.,Hunter, M.S.,Kassemeyer, S.,Kirian, R.A.,Kupitz, C.,Liang, M.,Lomb, L.,Malmerberg, E.,Martin, A.V.,Messerschmidt, M.,Nass, K.,Redecke, L.,Seibert, M.M.,Sjohamn, J.,Steinbrener, J.,Stellato, F.,Wang, D.,Wahlgren, W.Y.,Weierstall, U.,Westenhoff, S.,Zatsepin, N.A.,Boutet, S.,Spence, J.C.H.,Schlichting, I.,Chapman, H.N.,Fromme, P.,Neutze, R. (deposition date: 2013-10-09, release date: 2013-12-25, Last modification date: 2023-12-20)

Primary citation

Johansson, L.C.,Arnlund, D.,Katona, G.,White, T.A.,Barty, A.,DePonte, D.P.,Shoeman, R.L.,Wickstrand, C.,Sharma, A.,Williams, G.J.,Aquila, A.,Bogan, M.J.,Caleman, C.,Davidsson, J.,Doak, R.B.,Frank, M.,Fromme, R.,Galli, L.,Grotjohann, I.,Hunter, M.S.,Kassemeyer, S.,Kirian, R.A.,Kupitz, C.,Liang, M.,Lomb, L.,Malmerberg, E.,Martin, A.V.,Messerschmidt, M.,Nass, K.,Redecke, L.,Seibert, M.M.,Sjohamn, J.,Steinbrener, J.,Stellato, F.,Wang, D.,Wahlgren, W.Y.,Weierstall, U.,Westenhoff, S.,Zatsepin, N.A.,Boutet, S.,Spence, J.C.,Schlichting, I.,Chapman, H.N.,Fromme, P.,Neutze, R.
Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography.
Nat Commun, 4:2911-2911, 2013

PubMed Abstract: Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

PubMed: 24352554
DOI: 10.1038/ncomms3911

Experimental method

X-RAY DIFFRACTION (3.5 Å)