4C7R

Inward facing conformation of the trimeric betaine transporter BetP in complex with lipids

4C7R の概要

• エントリーDOI: 10.2210/pdb4c7r/pdb
• 分子名称: GLYCINE BETAINE TRANSPORTER BETP, CITRATE ANION, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: membrane protein, chemosensor and osmosensor, membrane, transport, cell membrane, secondary transporter, sodium coupled transport, transmembrane, betaine transport, hyperosmotic stress, anionic lipids
• 由来する生物種: CORYNEBACTERIUM GLUTAMICUM
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 191300.97

構造登録者

Koshy, C.,Yildiz, O.,Ziegler, C. (登録日: 2013-09-24, 公開日: 2013-10-30, 最終更新日: 2023-12-20)

主引用文献

Koshy, C.,Schweikhard, E.S.,Gaertner, R.M.,Perez, C.,Yildiz, O.,Ziegler, C.
Structural Evidence for Functional Lipid Interactions in the Betaine Transporter Betp
Embo J., 32:3096-, 2013

PubMed Abstract: Bilayer lipids contribute to the stability of membrane transporters and are crucially involved in their proper functioning. However, the molecular knowledge of how surrounding lipids affect membrane transport is surprisingly limited and despite its general importance is rarely considered in the molecular description of a transport mechanism. One reason is that only few atomic resolution structures of channels or transporters reveal a functional interaction with lipids, which are difficult to detect in X-ray structures per se. Overcoming these difficulties, we report here on a new structure of the osmotic stress-regulated betaine transporter BetP in complex with anionic lipids. This lipid-associated BetP structure is important in the molecular understanding of osmoregulation due to the strong dependence of activity regulation in BetP on the presence of negatively charged lipids. We detected eight resolved palmitoyl-oleoyl phosphatidyl glycerol (PG) lipids mimicking parts of the membrane leaflets and interacting with key residues in transport and regulation. The lipid-protein interactions observed here in structural detail in BetP provide molecular insights into the role of lipids in osmoregulated secondary transport.

PubMed: 24141878
DOI: 10.1038/EMBOJ.2013.226

実験手法

X-RAY DIFFRACTION (2.7 Å)